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Item #:
86986919
Phosphate buffered Saline (PBS)
Item #:
86986919
MUR 1887
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Phosphate buffered saline (PBS) is a commonly used solution in the cell culture manufacturing process and in the early stages of process development. It helps to maintain the pH of cell culture media by acting as a buffer.
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What Stands Out
pH Stability
PBS maintains a stable pH environment, crucial for biological experiments, ensuring accurate and reliable results across various applications, including cell culture and molecular biology.
Versatile Application
Ideal for diluting proteins and other biological samples, PBS is widely used in laboratories, making it an essential buffer for research across numerous scientific fields.
High Purity
Developed with high-purity ingredients, PBS is free from contaminants, ensuring high-quality results in sensitive experiments, which is essential for researchers seeking reliable data.
Product Details
| Package Weight | 2 Pound |
Who Should Buy?
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Cell Culture Researchers
PBS provides an isotonic solution ideal for maintaining the viability of cells during culture and experimental procedures.
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Molecular Biologists
Used to dilute samples and wash steps, PBS is crucial for various molecular biology techniques, such as PCR and Western blotting.
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Diagnostic Laboratories
PBS is commonly used in diagnostics to prepare reagents, buffers, and diluents necessary for various assay protocols.
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Animal Studies
PBS may not provide the required nutrients for animal cell growth or maintain physiological conditions in live animal studies.
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High Salt Experiments
Not suitable for experiments requiring high concentrations of salts, as it may interfere with results and cellular behavior.
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Long-Term Storage
PBS is not equipped for long-term cell storage; alternatives like cryoprotectants should be used to preserve cellular integrity.
Product Description
Phosphate buffered Saline (PBS)
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Customer Questions & Answers
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Question:
What is Phosphate Buffered Saline (PBS)?
Answer: Phosphate Buffered Saline (PBS) is a buffered saline solution that maintains a consistent pH and osmolarity, making it ideal for biological applications. It primarily consists of sodium chloride, potassium chloride, sodium phosphate, and potassium phosphate, which create a stable environment for cells and biological materials. PBS is commonly used in cell culture, molecular biology, and as a wash solution for various lab procedures to ensure that the treated cells remain healthy and viable. -
Question:
What are the main uses of PBS in laboratories?
Answer: PBS is widely used in laboratories for various applications, including cell culture, immunohistochemistry, and molecular biology experiments. It helps maintain osmotic balance and pH for cells cultured in vitro while also cleaning and diluting samples. These properties make PBS essential for preparing samples for analysis, washing away unbound antibodies in immunoassays, and serving as a diluent for reagents and staining solutions. -
Question:
Is PBS suitable for all types of cell cultures?
Answer: PBS is generally suitable for various mammalian cell cultures, as it provides a stable environment. However, specific cell lines may have unique requirements regarding growth media that PBS alone cannot fulfill. It’s often used as a wash or dilution solution rather than a growth medium. For optimal results, it is advisable to consult the culture manuals or protocols specific to your cell line to ensure appropriate conditions. -
Question:
How should PBS be stored?
Answer: PBS should be stored in a cool, dry place away from direct sunlight. If prepared fresh, it’s typically stable at room temperature for a short duration. For prolonged storage, it’s best kept at 4°C. Avoid repeated freeze-thaw cycles, as they can degrade the components of the solution. Proper storage practices help maintain the integrity of the buffer, ensuring reliable performance in experimental procedures. -
Question:
Can PBS be used for in vivo studies?
Answer: While PBS is primarily used in vitro for cell culture and laboratory applications, it can also be utilized in in vivo studies as a saline solution to maintain fluid and electrolyte balance. However, in such cases, it is essential to consider the specific requirements of the study and how PBS interacts with biological systems to ensure the validity of the results. -
Question:
What precautions should I take when handling PBS?
Answer: When handling PBS, wear appropriate personal protective equipment (PPE) such as gloves and safety glasses to prevent any potential skin or eye irritation. Follow standard laboratory safety protocols to minimize contamination. Additionally, always use sterile techniques to prepare solutions, especially when working with cell cultures, to prevent unwanted contamination and ensure the accuracy of your experiments. -
Question:
How is PBS prepared in the lab?
Answer: PBS can be prepared by dissolving the appropriate amounts of sodium chloride, potassium chloride, and phosphate buffers in distilled water. The exact recipe may vary slightly depending on the desired pH and osmolarity. Alternatively, PBS is also available in ready-to-use formulations, which can save time in the lab. Following proper preparation protocols is important to maintain the buffer's effectiveness for sensitive applications. -
Question:
What is the pH range of PBS, and why is it important?
Answer: PBS typically has a pH range of about 7.2 to 7.4, which is crucial for maintaining cellular functions during experimental procedures. A stable pH ensures optimal conditions for enzymatic reactions, protein stability, and overall cell health. Deviations from this pH can negatively impact biological experiments, so the buffering capacity of PBS helps to prevent such fluctuations, making it a reliable choice for laboratory use. -
Question:
Can I use PBS for washing biological samples?
Answer: Yes, PBS is commonly used for washing biological samples, particularly in immunoassays and cell culture. Its isotonic and buffered nature helps to maintain cell integrity while effectively removing unbound substances, such as antibodies. This cleaning step is essential to minimize background noise in assays and enhance the accuracy of results, making PBS an invaluable component of many laboratory protocols. -
Question:
Where can I buy Phosphate Buffered Saline (PBS) in Mauritius?
Answer: You can buy Phosphate Buffered Saline (PBS) in Mauritius from Ubuy. They offer a range of PBS products suitable for laboratory use, ensuring you have access to quality solutions for your scientific needs. Ubuy provides a reliable platform where you can find the specific formulations you require for your research or experimentation.
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Features & Benefits
- PBS is a vital solution in cell culture manufacturing
- Maintains pH of cell culture media by acting as a buffer
- Versatile usage: washing cells, transporting cells, diluting cells for counting, and preparing reagents
- Formulated without calcium and magnesium
- Produced in a cGMP-compliant facility with stringent quality control measures
- Filtered through a 0.1 μm sterile filter and tested for sterility, pH, osmolality, and endotoxin
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